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Seattle Children’s Hospital Research Scientist I (CIIT) in Seattle, Washington

Overview:The Acharya lab (www. seattlechildrens. org/directory/mridu-acharya) at Seattle Children's Research Institute is hiring a scientist to join a rapidly expanding research program on understanding signaling mechanisms regulating immune responses. Research in our group is focused on studying mechanisms that lead to efficient antibody response to viruses and how these get dysregulated during development of autoimmunity. We also collaborate with the James lab and Rawlings lab at Seattle Children's to used gene editing technology in B cells to manipulate genes and study changes in antibody responses For this position, we are seeking a highly motivated scientist to work with the group in performing experiments related to flow cytometry, ELISA, western blots and tissue culture experiments, and experiments involving immunization of mice and harvesting target tissues from mice. Additionally, the applicant will be responsible for data anlalysis, presentation of results and working together with other scientists in the group.Key responsibilities:Design experiments to perform flow cytometry, western blot.Design experiments to study activation of signaling proteins in B cellsPerform ELISA on serum samples acquired from mice to study antibody responseAssist with genotyping and management of mouse colonyImmunize mice with different viral antigens Perform experiments on tissues harvested from mice. Analyse data and present data at group meetings. Contribute to writing for publication of results; generate figures for papers and grants.Requirements- Bachelor's Degree in biology, microbiology, immunology, molecular biology or biochemistry.- Prior experience in a biology laboratory setting.Preferred:- Research experience in biochemistry, molecular biology, and tissue culture techniques is highly preferred.Our Commitment to DiversityOur community welcomes diverse experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our increasingly diverse patients and families. Our organization recruits, employs, trains, compensates, and promotes without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics. The people who work at Seattle Children’s are members of a community that seeks to respect and celebrate all the qualities that make each of us unique. Each of us is empowered to be ourselves within this community, which cultivates and promotes equity, diversity, and inclusion at all levels. Seattle Children’s is proud to be an Equal Opportunity Workplace and Affirmative Action Employer.

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