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University of Florida Biological Scientist III - Pediatrics Cellular Molecular Therapy in Gainesville, Florida

Biological Scientist III - Pediatrics Cellular Molecular Therapy

Apply now ( Job no: 519962

Work type: Staff Full-Time

Location: Main Campus (Gainesville, FL)

Categories: Agriculture/Life Science/Vet Medicine, Other, Research/Scientific/Grants

Department: 29092300 - MD-PEDS-CELL / MOLEC THERP DIV

Classification Title:

Biological Scientist III

Job Description:

The Biological Scientist III position described herein performs research for the Department of Pediatrics, Division of Infectious Disease, Dr. Bhaduri-McIntosh Research Laboratory. The incumbent will perform laboratory experiments to investigate viral and cellular DNA replication and repair in lytic and latent EBV-cancer cells using cutting edge assays of iPOND, DNA molecular combing, mass spectrometry, etc. using well-established protocols. Once trained, the individual in this position is expected to function under minimal supervision to perform work using established methods. The position requires work associated with live BSL2 viruses, viral vector production, molecular biology, cell biology, and cell culture maintenance.

This is a senior level Biological Scientist position associated with investigation of a cancer-causing virus. The Biological Scientist is expected to be able to:

(1) follow pre-established guidelines and procedures to maintain suspension and adherent cell cultures;

(2) complete the execution of assigned experiments;

(3) maintain accurate laboratory records, summaries, and notes;

(4) present findings to the PI at laboratory meetings;

(5) follow safety procedures associated with the laboratory;

(6) and maintain a computer database.

The Biological Scientist III must be able to perform the following essential functions:

Investigate mechanisms by which EBV rewires cellular DNA replication: These experiments will involve cell culture, transfection, cloning, cell cycle analysis, PCR, RT-qPCR, immunoblotting, immunoprecipitation, fluorescence imaging, and flow cytometry.

Investigate viral genome replication during the lytic phase of EBV: These experiments will involve the use of similar types of assays (described above).

Guide and train undergraduate and graduate researchers.

Maintaining and updating laboratory notebooks and protocols.

Maintain laboratory equipment and order supplies as needed.

Advertised Salary:

$54,000 - $57,000 annually; commensurate based on qualifications and experience.

Minimum Requirements:

Bachelor’s degree in biology or a closely related field and seven years of relevant experience; or a Master’s degree in biology or a closely related field and five years of relevant experience; Doctoral degree in biology or a closely related field and two years of relevant experience.

Preferred Qualifications:

Working knowledge of basic laboratory techniques.

Training in virology, preferably herpesvirology; proficiency in adherent and suspension cell cultures,

competence in transfection, qPCR, RT-PCR, cloning, flow cytometry, immunofluorescence,

immunoblotting, immunoprecipitation, chromatin immunoprecipitation, virus mutagenesis, proximity ligation

assays, iPOND, DNA molecular combing, transcriptomics, mass spectrometry, and CRISPR-Cas9


Special Instructions to Applicants:

To be considered for this position, please upload your cover letter of letter of interest, resume, and a list of three professional references.

Normal work hours are 8:00am to 5:00pm, Monday through Friday with daily scheduling when necessary due to pending experiment(s).

This is a time limited position.

The University of Florida is committed to non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, gender identity and expression, marital status, national origin, political opinions or affiliations, genetic information and veteran status in all aspects of employment including recruitment, hiring, promotions, transfers, discipline, terminations, wage and salary administration, benefits, and training.

Application must be submitted by 11:55 p.m. (ET) of the posting end date.

Health Assessment Required:Yes

Advertised: 14 Jan 2022 Eastern Standard Time

Applications close: 28 Jan 2022 Eastern Standard Time