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Children's Hospital Boston Research Assistant I - Sahin Lab in Boston, Massachusetts

61212BRTitle:Research Assistant I - Sahin LabDepartment:NeurologyAutoReqId:61212BRStatus:Full-TimeStandard Hours per Week:40 Job Category:ResearchJob Posting Description:At Boston Children’s Hospital, the quality of our care – and our inclusive hospital working environment – lies in the diversity of our people. With patients from local communities and 160 countries around the world, we’re committed to reflecting the spectrum of their cultures, while opening doors of opportunity for our team. Here, different talents pursue common goals. Voices are heard and ideas are shared. Join us, and discover how your unique contributions can change lives

A research assistant position is available for a start date in Winter 2021-2022 with a flexible start date for within the Sahin laboratory at Boston Children’s Hospital. The individual will join a team that utilizes a mouse model to study tuberous sclerosis complex (TSC), and more specifically the role of interneurons in TSC-associated epilepsy. The research assistant will be trained to maintain the mouse colony, genotyping, DNA and RNA isolation, western blots and characterize these animals using cutting-edge technologies. The candidate will also have the opportunity to actively participate in meeting discussions and presentations.

Duties & Responsibilities:

  • Mouse colony maintenance- setting up matings, toeing, weaning, weighing animals.

  • Genotyping of mice

  • Characterization of mice- analysis of behavioral paradigms, confocal microscopy

  • Isolation and analyzing RNA/DNA and proteins, including but not limited to: PCR,

  • Immunoprecipitation, immunocytochemistry, western blot.

  • Lab maintenance, i.e. ordering supplies, general upkeep, etc.

  • Organizing processed samples.

Qualifications:

  • A BA/BS degree in Biology/Molecular Biology/Biochemistry/Neurobiology or related

  • field.

  • Flexible working hours during weekdays AND weekends on rotating schedule.

  • Experience with animals required; experience with genotyping is a plus

  • Past research experience in cell biology, molecular biology, genetics, or neuroscience at the undergraduate level or beyond, preferred.

  • Excellent communication, organization, time management, and record-keeping skills a must.

Please note: During a public health emergency, individuals in this role may be expected to take on additional duties to respond to organizational needs.

BCH offers competitive compensation and benefits, including a rotating days/evenings and week-end schedule, affordable health, vision and dental insurance, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition Reimbursement, and discounted rates on T-passes (50% off).

Boston Children’s Hospital is an Equal Opportunity / Affirmative Action Employer. Qualified applicants will receive consideration for employment without regard to their race, color, religion, national origin, sex, sexual orientation, gender identity, protected veteran status or disability.Office/Site Location:BostonRegular, Temporary, Per Diem:Regular

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